I’m so sorry about your rooster by the way 😔 :hugs.
I actually recently incubated seven eggs and hatched out some chicks for a friend whose entire flock except four hens had gotten wiped out by a coyote. They’d collected eggs for about a week, but I think they could’ve gone even longer, because...
Hello!
I could be wrong, but I believe that a hen will continue to lay fertile eggs at least a week after mating with a rooster. Try to continue to collect the eggs and put them in the incubator when you’ve collected about a week’s worth. Best of luck!