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There is no use in vaccinating older birds and even the chicks need to be vaccinated right after hatch and kept separate/quarantined away from older birds and their dust for weeks, better months to develop some kind of immunity.

I am sorry for your losses. Did you ever send any of the deceased hens in for a professional necropsy? Even supposing it is Marek's you are dealing with, there is no use in culling your broody as the virus is ubiquitous and will last for years in the ground, dust etc. Just let her hatch the...