This is a great idea, one I've often utilized in my own flock. I do have one hen who just has to live by herself now, as the three hens who pick on her don't give her a break, but this method has worked with some of my others.
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You may not have room in your run to temporarily fence off one corner, but you might be able to find a corner in the coop for it. Or perhaps you've been meaning to expand your run and just need some motivation. This is a good time to take a look at your setup and think about pushing out a fence to make 100 more square feet of space to make your flock happier. In a pinch, a dog crate large enough for the bully victim to stand up in can serve as a safe refuge during bully victim treatment. It only needs to be used during the day as the victim will roost with the others at night.