Yes, all my hives have one 3/4" hole in the upper brood box "below" the handhold. I leave them open all year, winter bees will use the hole for cleansing flights. The holes in your hive bodies are in the handhold so be careful when lifting the boxes, you dont want to crush bees on your glove when lifting. Just give them a puff of smoke before grabbing the handhold.I'm guessing the holes are extra "doorways" for the bees. So I'm assuming they should go in front...?